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The results were subsequently screened by title and abstract, and only studies exploring the relationship between cholesterol and synaptic function were included

Data normality was evaluated by Kolmogorov-Smirnov test, and comparisons between groups were performed with One-way ANOVA (in mice experiments) or Two-way ANOVA (in cell experiments) with Bonferroni post-hoc test

Sex as a biological variable Male and female mice were used as they became available in the breeding colony and in litters of embryos and fetuses recovered

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